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Identification of the IFN-stimulated genes in IFN-α2b-treated intrahepatic cholangiocarcinoma cells

GSE252095 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/01/20 Platform GPL11154
Summary
Interferon-α2b (IFN-α2b) is a highly active cytokine protein that belongs to the interferon-α (IFN-α) family. As a pleiotropic cytokine, IFN-α2b has beneficial antiviral, antitumour, antiparasitic, and immunomodulatory activities. In vivo and in vitro, IFN-α2b can activate the JAK-STAT pathway (also known as IFN-I pathway) and function by inducing the transcription of downstream IFN-stimulated genes (ISGs).To identify the ISGs in intrahepatic cholangiocarcinoma (ICCA) cells and evaluate the therapeutic value of IFN-α2b, ICCA cells were treated with recombinant IFN-α2b protein. Total RNA samples were extracted and used for RNA-seq. Certain ISGs (e.g., MXs, IFITs, OASs) were found to be significantly upregulated upon IFN-α2b treatment.
Published in
Unveiling the role of HP1α-HDAC1-STAT1 axis as a therapeutic target for HP1α-positive intrahepatic cholangiocarcinoma
Xiong F, Wang D, Xiong W et al. · Journal of experimental & clinical cancer research : CR 2024 · PMID 38812060 · doi:10.1186/s13046-024-03070-3
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Direct links to NCBI, no account and no request form: the whole study as GSE252095_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1057779 and SRA study SRP480276. Searching any of these in the dataset finder brings you back here.

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