C. difficile's proline reductase harnesses host and commensal factors to drive efficient metabolism and progression to disease. Commensal plus C. difficile.

Gnotobiotic mice were colonized with Clostridium sardiniense DSM 599 7 days prior to infection with 10^3 spores of wild-type Clostridioides difficile ATCC 43255 or the proline reductase (ΔprdB) mutant of ATCC43255 strain. Cecal contents were collected and underwent RNASeq at the timepoints indicated below for pathway enrichment and transcritpmic analyses. Metatranscriptomic sequence processing was carried out as in Girinathan, et al, 2021 (PMID: 34637781)