GEO series

Androgens inhibit protective CD8+ T cell responses against pre-erythrocytic malaria parasites

GSE253755 Mus musculus (mouse) RNA-seq (gene expression) 40 samples Submitted 2025/04/04 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 40 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 40 samples: 10 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Androgens inhibit protective CD8(+) T cell responses against pre-erythrocytic malaria parasites in miceNature communications · 2025

Summary (written by the submitters)

Attenuated whole organism vaccines targeting the malaria liver stage reliably confer sterile immunity. These vaccines can completely protect female mice from infection, but protection in male mice remains unproven. We discovered that male BALB/cJ mice vaccinated with prime-and-trap, a whole organism-based vaccine strategy, exhibit poorer protection against Plasmodium yoelii sporozoite challenge than females.

Read the full summary ↓

We investigated this sex difference, and identified vaccinated males have fewer hepatic memory CD8+ T cells than females when scaling for differences in liver biomass, and reduced acute inflammatory responses post-vaccination. Surgical hormone manipulation clarified that the presence of testicular hormones, not the absence of ovarian hormones, hindered protection in male mice. The presence of androgens did not affect memory CD8+ T cells quantity nor quality of, but inhibited protective cellular responses during sporozoite challenge. Thus, both males and females form functional memory responses following prime-and-trap vaccination, but androgens during sporozoite challenge impair protection in male mice.

Published in

Androgens inhibit protective CD8(+) T cell responses against pre-erythrocytic malaria parasites in mice

Duncombe CJ, Sen N, Watson FN et al. · Nature communications 2025 · PMID 40467616 · doi:10.1038/s41467-025-60193-7

This dataset

Open GSE253755 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE253755_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1066874 and SRA study SRP484809. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40467616) ↗

Study design

10 conditions, mostly with about 4 replicates each

Male, RAS, +44 hours, ×4 Male, mock, ×4 Male, RAS, +6 days, ×4 Male, P&T, +44 hours, ×4 Male, P&T, +6 days, ×4 Female, P&T, +6 days, ×4 Female, mock, ×4 Female, RAS, +44 hours, ×4 +2 more

Supports a between-group comparison across 40 samples.

How we read this: 10 replicated groups read from 40 sample titles; they account for 40 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8027491 Male, RAS, +44 hours, 4726 Male, RAS, +44 hours,
GSM8027492 Male, RAS, +44 hours, 4727 Male, RAS, +44 hours,
GSM8027493 Male, RAS, +44 hours, 4728 Male, RAS, +44 hours,
GSM8027494 Male, RAS, +44 hours, 4729 Male, RAS, +44 hours,
GSM8027495 Male, mock, 4745 Male, mock,
GSM8027496 Male, RAS, +6 days, 4746 Male, RAS, +6 days,
GSM8027497 Male, RAS, +6 days, 4747 Male, RAS, +6 days,
GSM8027498 Male, RAS, +6 days, 4748 Male, RAS, +6 days,
GSM8027499 Male, RAS, +6 days, 4749 Male, RAS, +6 days,
GSM8027500 Male, mock, 4750 Male, mock,
GSM8027501 Male, P&T, +44 hours, 4751 Male, P&T, +44 hours,
GSM8027502 Male, P&T, +44 hours, 4752 Male, P&T, +44 hours,
GSM8027503 Male, P&T, +44 hours, 4753 Male, P&T, +44 hours,
GSM8027504 Male, P&T, +44 hours, 4754 Male, P&T, +44 hours,
GSM8027505 Male, mock, 4755 Male, mock,
GSM8027506 Male, P&T, +6 days, 4756 Male, P&T, +6 days,
GSM8027507 Male, P&T, +6 days, 4757 Male, P&T, +6 days,
GSM8027508 Male, P&T, +6 days, 4758 Male, P&T, +6 days,
GSM8027509 Male, P&T, +6 days, 4759 Male, P&T, +6 days,
GSM8027510 Male, mock, 4760 Male, mock,
GSM8027511 Female, P&T, +6 days, 4776 Female, P&T, +6 days,
GSM8027512 Female, P&T, +6 days, 4777 Female, P&T, +6 days,
GSM8027513 Female, P&T, +6 days, 4778 Female, P&T, +6 days,
GSM8027514 Female, P&T, +6 days, 4779 Female, P&T, +6 days,
GSM8027515 Female, mock, 4780 Female, mock,
GSM8027516 Female, RAS, +44 hours, 4781 Female, RAS, +44 hours,
GSM8027517 Female, RAS, +44 hours, 4782 Female, RAS, +44 hours,
GSM8027518 Female, RAS, +44 hours, 4783 Female, RAS, +44 hours,
GSM8027519 Female, RAS, +44 hours, 4784 Female, RAS, +44 hours,
GSM8027520 Female, mock, 4785 Female, mock,
GSM8027521 Female, RAS, +6 days, 4786 Female, RAS, +6 days,
GSM8027522 Female, RAS, +6 days, 4787 Female, RAS, +6 days,
GSM8027523 Female, RAS, +6 days, 4788 Female, RAS, +6 days,
GSM8027524 Female, RAS, +6 days, 4789 Female, RAS, +6 days,
GSM8027525 Female, mock, 4790 Female, mock,
GSM8027526 Female, P&T, +44 hours, 4791 Female, P&T, +44 hours,
GSM8027527 Female, P&T, +44 hours, 4792 Female, P&T, +44 hours,
GSM8027528 Female, P&T, +44 hours, 4793 Female, P&T, +44 hours,
GSM8027529 Female, P&T, +44 hours, 4794 Female, P&T, +44 hours,
GSM8027530 Female, mock, 4795 Female, mock,

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