Morphine-responsive neurons that regulate mechanical antinociception
Single-cell RNA-seq · mouse · 21 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 21 samples: 7 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Morphine-responsive neurons that regulate mechanical antinociceptionScience (New York, N.Y.) · 2024
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE253816_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1067304 and SRA study SRP485024. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 21 samples.
How we read this: 7 replicated groups read from 21 sample titles; they account for 21 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8028274 RVM, Reference 1 RVM, Reference
- GSM8028275 RVM, Reference 2 RVM, Reference
- GSM8028276 RVM, Reference 3 RVM, Reference
- GSM8028277 Descending 1 Descending
- GSM8028278 Descending 2 Descending
- GSM8028279 Descending 3 Descending
- GSM8028280 Arc Control1 Arc Control
- GSM8028281 Arc Control2 Arc Control
- GSM8028282 Arc Control3 Arc Control
- GSM8028283 Arc Morphine1 Arc Morphine
- GSM8028284 Arc Morphine2 Arc Morphine
- GSM8028285 Arc Morphine3 Arc Morphine
- GSM8028286 Spinal Cord, shScrambled1 Spinal Cord, shScrambled
- GSM8028287 Spinal Cord, shScrambled2 Spinal Cord, shScrambled
- GSM8028288 Spinal Cord, shScrambled3 Spinal Cord, shScrambled
- GSM8028289 Spinal Cord, shBDNF1 Spinal Cord, shBDNF
- GSM8028290 Spinal Cord, shBDNF2 Spinal Cord, shBDNF
- GSM8028291 Spinal Cord, shBDNF3 Spinal Cord, shBDNF
- GSM8028292 Spinal Cord, no shRNA1 Spinal Cord, no shRNA
- GSM8028293 Spinal Cord, no shRNA2 Spinal Cord, no shRNA
- GSM8028294 Spinal Cord, no shRNA3 Spinal Cord, no shRNA
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- GSE336091 KMT2C catalytic activity regulates enhancer H3K4me1 and synaptic gene expression in cortical excitatory neurons [CUT&Tag] 48 samples shares regulate, neurons
- GSE241770 ACSS2 mediates an epigenetic pathway to regulate maternal Beta-cell adaptation and progression of gestational diabetes (scRNA-Seq) 7899 samples shares regulate
- GSE271100 Stem cells tightly regulate dead cell clearance to maintain tissue fitness [scRNA-seq] 1037 samples shares regulate
- GSE286046 Asymmetric Histone Inheritance Regulates Differential Transcription Re-initiation and Cell Fate Decisions in Mouse Olfactory Horizontal Basal Cells 96 samples shares regulate
- GSE222996 The immune landscape of the “deregulated host response” in sepsis: newly emerging MDSCs with high expression of CD47 leads to B cell suppression via App-CD74 signaling [RNA-seq] 61 samples shares regulate
- GSE311705 Uterine epithelial ERα regulates preimplantation uterine luminal epithelial (LE) and uterine mRNAs 59 samples shares regulate
- GSE223424 Immune checkpoint TIM-3 regulates microglia and Alzheimer’s disease 57 samples shares regulate
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.