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Investigating DDX41's Functional Role in Erythroid G-Quadruplex Dissolution

GSE254143 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/08/13 Platform GPL19057
Summary
To explore the functional role of DDX41 in the dissolution of G-quadruplexes, we conducted Cut&Run assays in both mouse erythroid cells and hematopoietic stem and progenitor cells (HSPCs). Initially, we harvested mouse bone marrow cells through PBS flushing. Subsequently, we isolated erythroid cells using Ter119 antibodies and purified HSPCs by selectively collecting lineage-negative cells. These cells were then subjected to Cut&Run assays, utilizing DDX41 antibodies and Bg4 antibodies. The DNA fragments obtained through this process were sonicated and sent for sequencing and subsequent analysis via NUseq.
Published in
DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death
Bi H, Ren K, Wang P et al. · Nature communications 2025 · PMID 40764304 · doi:10.1038/s41467-025-62307-7
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Also filed as BioProject PRJNA1068623 and SRA study SRP485751. Searching any of these in the dataset finder brings you back here.

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