← BioTransfer GEO Dataset Finder
GEO series

Transcriptomic Signatures of Neuronally-Derived Extracellular Vesicles Reveals Presence of Olfactory Receptors in Clinical Samples of Traumatic Brain Injury Patients

GSE254880 Homo sapiens (human) RNA-seq (gene expression) 16 samples Submitted 2024/02/29 Platform GPL20301
At a glance

RNA-seq (gene expression) · human · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
16 samples: 8 × Control_Sample vs 8 × STBI_SampleGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Transcriptomic Signatures of Neuronally Derived Extracellular Vesicles Reveal the Presence of Olfactory Receptors in Clinical Samples from Traumatic Brain Injury PatientsInternational journal of molecular sciences · 2024
Summary (written by the submitters)
Traumatic brain injury (TBI) is one of the major causes of death and disability worldwide. Recently, the FDA has approved blood-based biomarkers, GFAP and UCH-L1, to evaluate the need for CT scans, however, clinical translation is currently limited. Exosomes are small microcellular vesicles which are released from the cell in both normal and diseased states, as part of extracellular communication.
Read the full summary ↓
The aim of this study was to isolate neuronal exosomes derived from the serum of patients with severe TBI and characterize the messenger RNA content of the exosomes released by the injured neurons in order to identify new potential blood-based biomarkers. RNA was isolated from neuronal exosomes and total transcriptomic sequencing was performed. RNA sequencing data from neuronal exosomes isolated from serum showed mRNA transcripts of several neuronal genes. Additionally, the RNA sequencing data revealed mRNA of several olfactory receptor genes, which were present at elevated concentrations in the neuronal exosomes. Further validation in serum samples of mild TBI patients revealed a similar upregulation of olfactory receptors. The data from these experiments suggests that damage to the neurons in the olfactory neuroepithelium and in the brain, following a TBI, may cause the release of mRNA from these receptors in the exosomes. Thus, olfactory receptors can be further investigated as potential biomarkers for TBI diagnosis.
Published in
Transcriptomic Signatures of Neuronally Derived Extracellular Vesicles Reveal the Presence of Olfactory Receptors in Clinical Samples from Traumatic Brain Injury Patients
Bhomia M, Feng Y, Deleon P et al. · International journal of molecular sciences 2024 · PMID 38474024 · doi:10.3390/ijms25052777
This dataset
Open GSE254880 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE254880_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1072136 and SRA study SRP487529. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 38474024) ↗
Study design
8 × Control_Sample vs 8 × STBI_Sample

Supports a between-group comparison across 16 samples.

How we read this: 2 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.