Study of microbial species in extracellular vesicles of sclerosis multiple patients

Extracellular vesicles (EVs) are released by most cell types and are implicated in several biological and pathological processes, including multiple sclerosis (MS). In this study we performed RNA sequencing to analyze the diversity of microorganisms by assignment of reads using different taxa profilers. To diminish the risk of false positive biases derived from sample handling, we performed a similar analysis on EVs derived from known cultured bacterial species, as well as artificially-generated samples. Overall, we detect a range of microbial species in MS and healthy control (HC) samples, that are not detected in control samples, as well as species with differential abundance between MS and HC samples. These results reveal the relevance of putative communication of microbial species using EVs as a communication vector.