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Identification of potential genomic targets of CDCA7

GSE255395 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/07/31 Platform GPL17021
Summary
CDCA7, encoding a protein with a C-terminal cysteine-rich domain (CRD), is mutated in immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, a disease related to hypomethylation of peri/centromeric satellite DNA. Previous work suggests that the CDCA7 CRD is implicated in DNA binding, which plays a key role in directing the DNA methylation mechinery to peri/centromeric regions. To identify potential genomic targets of CDCA7, we performed ChIP-Seq using CDCA7 knockout (KO) mouse embryonic stem cells (mESCs) stably expressing HA-tagged wild-type (WT) or ICF mutant (R285H) mCDCA7.
Published in
The ICF syndrome protein CDCA7 harbors a unique DNA binding domain that recognizes a CpG dyad in the context of a non-B DNA
Hardikar S, Ren R, Ying Z et al. · Science advances 2024 · PMID 39178265 · doi:10.1126/sciadv.adr0036
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Direct links to NCBI, no account and no request form: the whole study as GSE255395_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1074800 and SRA study SRP489000. Searching any of these in the dataset finder brings you back here.

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