GEO series

Next generation sequencing from primary glioblastoma patient tissues (Total RNA-seq)

GSE255626 Homo sapiens (human) RNA-seq (gene expression) 109 samples Submitted 2026/01/21 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 109 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Topic: GlioblastomaThis series is part of our curated briefing on it.
Samples: 109 samples: 67 × primary GBM patient vs 2 × primary GBM patient 52 peripheral regionGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Inhibition of FOS-Like Antigen 1 Reduces Chemoresistance to Temozolomide Through Stemness Reprogramming via IL-6/STAT3(Tyr705) PathwayMedComm · 2026

Summary (written by the submitters)

To investigate transcriptome landscape in primary GBM, we performed total RNA-seq using 72 tumor tissues from 52 patients with primary GBM.

Published in

Inhibition of FOS-Like Antigen 1 Reduces Chemoresistance to Temozolomide Through Stemness Reprogramming via IL-6/STAT3(Tyr705) Pathway

Sim J, Park J, Heo J et al. · MedComm 2026 · PMID 41556041 · doi:10.1002/mco2.70593

This dataset

Open GSE255626 in finder → Find more human RNA-seq datasets → Glioblastoma landscape →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE255626_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1075795 and SRA study SRP489659. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41556041) ↗

Study design

67 × primary GBM patient vs 2 × primary GBM patient 52 peripheral region

Supports a between-group comparison across 69 samples.

How we read this: 2 replicated groups read from 109 sample titles; they account for 69 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8076242 primary GBM patient 3 primary GBM patient
GSM8076243 primary GBM patient 4 primary GBM patient
GSM8076244 primary GBM patient 5 primary GBM patient
GSM8076245 primary GBM patient 6 primary GBM patient
GSM8076246 primary GBM patient 7 primary GBM patient
GSM8076247 primary GBM patient 10 primary GBM patient
GSM8076248 primary GBM patient 11 primary GBM patient
GSM8076249 primary GBM patient 12 primary GBM patient
GSM8076250 primary GBM patient 13 primary GBM patient
GSM8076251 primary GBM patient 14 primary GBM patient
GSM8076252 primary GBM patient 15 primary GBM patient
GSM8076253 primary GBM patient 18 primary GBM patient
GSM8076254 primary GBM patient 20 primary GBM patient
GSM8076255 primary GBM patient 21 primary GBM patient
GSM8076256 primary GBM patient 22 primary GBM patient
GSM8076257 primary GBM patient 23 primary GBM patient
GSM8076258 primary GBM patient 24 primary GBM patient
GSM8076259 primary GBM patient 25 primary GBM patient
GSM8076260 primary GBM patient 26 primary GBM patient
GSM8076261 primary GBM patient 27 primary GBM patient
GSM8076262 primary GBM patient 28 primary GBM patient
GSM8076263 primary GBM patient 29 primary GBM patient
GSM8076264 primary GBM patient 31 primary GBM patient
GSM8076265 primary GBM patient 32 primary GBM patient
GSM8076266 primary GBM patient 34 primary GBM patient
GSM8076267 primary GBM patient 35 primary GBM patient
GSM8076268 primary GBM patient 36 primary GBM patient
GSM8076269 primary GBM patient 37 tumor core region
GSM8076270 primary GBM patient 37 peripheral region
GSM8076271 primary GBM patient 38 tumor core region
GSM8076272 primary GBM patient 38 peripheral region
GSM8076273 primary GBM patient 39 tumor core region
GSM8076274 primary GBM patient 39 peripheral region
GSM8076275 primary GBM patient 40 primary GBM patient
GSM8076276 primary GBM patient 41 peripheral region
GSM8076277 primary GBM patient 41 subventricular zone
GSM8076278 primary GBM patient 41 tumor core region
GSM8076279 primary GBM patient 42 cortex
GSM8076280 primary GBM patient 42 tumor core region
GSM8076281 primary GBM patient 42 subventricular zone

+ 69 more — browse all 109 samples with per-sample file links →

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Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.