GEO series
Multigenerational cell tracking of DNA replication and heritable DNA damage
GSE255874
Homo sapiens
Expression profiling by high throughput sequencing
10 samples
2025/03/31
GPL18573
Summary
The goal of this project was to analyze gene expression changes upon polyploidization. To this end, human U-2 OS cells were exposed to the NEDD8-activating enzyme (NAE) inhibitor Pevonedistat (MLN4924), a first-in-class anticancer drug that induces DNA re-replication, or to oncogenic H-RAS V12 (HRAS) over-expression. Untreated U-2 OS cells served as control. Cells with 2N-4N DNA content were separated from cells with >4N DNA content by FACS and single cells were sorted into 384 well plates for scRNA-seq analysis.
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Paper (PMID 40399682) ↗
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