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Renal cancer cells secrete exosomal ncRNAs that affect tumor microenvironment

GSE256016 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/01/08 Platform GPL18573
Summary
Cancer cells secrete multiple molecules that affect tumor microenviroment. In our previous study we analyzed the secretory proteome and metabolome of renal cancer cells (PMID: 36604669). Small non-coding RNAs (sncRNAs) encompass a variable group of oligonucleotides, including miRNAs, piRNAs, and tRNA-derived fragment that can affect the expression of target genes. Here, we aimed to analyzed sncRNAs that are secreted by renal cancer cells and can affect tumor microenvironment. To this end, we: i) collected the conditioned media (CM) from two renal cancer-derived cell lines, Caki-1 and KIJ265T, as well as RPTEC, the control non-tumorous kidney cell line derived from proximal tubules; ii) used the CM for isolation of exosomal total RNA; iii) performed RNAseq to target and analyze the profiles of sncRNAs. We identified multiple miRNAs, piRNAs and tRNAs that were differently secreted by renal cancer cells when compared with non-tumorous proximal tubules. The analysis was followed by qPCR validation and functional analysis to reveal sncRNAs that affect tumor microenvironment.
Published in
Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cells
Bogusławska J, Grzanka M, Popławski P et al. · Cell communication and signaling : CCS 2025 · PMID 39754169 · doi:10.1186/s12964-024-02001-1
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Also filed as BioProject PRJNA1077387 and SRA study SRP490098. Searching any of these in the dataset finder brings you back here.

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