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Transcriptomic and V(D)J profiling at the single cell level of plasmablasts from lymph nodes of mice immunized with bMOG protein

GSE260585 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2024/03/04 Platform GPL24247
Summary
Autoantibodies contribute to many autoimmune diseases, yet there is no therapy to neutralize them selectively. A popular mouse model, experimental autoimmune encephalomyelitis (EAE), could serve to develop such a therapy, provided we can better understand the nature and importance of the autoantibodies involved. In this study, we analyzed autoantibody-secreting extrafollicular plasmablasts in mice with EAE induced by immunization with a mutated myelin oligodendrocyte glycoprotein (MOG) antigen called bMOG. These CD138+ cells were enriched from lymph nodes at day 8 post-immunization and analyzed by single-cell RNA sequencing using 10× Genomics technologies. Here we provide the raw and processed data obtained from the gene expression (GEX) and VDJ cDNA libraries.
Published in
Turncoat antibodies unmasked in a model of autoimmune demyelination: from biology to therapy
Taghipour-Mirakmahaleh R, Morin F, Zhang Y et al. · bioRxiv : the preprint server for biology 2024 · PMID 39677612 · doi:10.1101/2024.12.03.623846
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Direct links to NCBI, no account and no request form: the whole study as GSE260585_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1082281 and SRA study SRP492588. Searching any of these in the dataset finder brings you back here.

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