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Effect of rocking culture on keratinocyte differentiation in three-dimensional reconstitution human epidermis

GSE260705 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/06/01 Platform GPL24676
Summary
A three-dimensional culture system of keratinocytes is able to mimic the formation of stratified epidermis. Air-exposure treatment is essential to promote keratinocyte differentiation. During the culture, we compared the transcripts in the cells with (Air) and without (Liq; submerged) air-exposure. Then, since rocking (shaking) the culture dish enabled to recover the keratinocyte differentiation despite the submerged culture (Liq), a comparative study between with and without rocking was also carried out to investigate the characteristics of the restored differentiation by RNA-seq. Transcriptome analyses revealed the expected similar pattern of the transcripts between air-exposure and rocking culture, which differed from those in Liq culture.
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Also filed as BioProject PRJNA1082734 and SRA study SRP492854. Searching any of these in the dataset finder brings you back here.

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