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Chronic opioid treatment arrests neurodevelopment and alters synaptic activity in human midbrain organoids

GSE260711 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2024/03/05 Platform GPL24676
Summary
Understanding the impact of long-term opioid exposure on the embryonic brain is critical due to the surging number of pregnant mothers with opioid dependency, but has been limited by human brain inaccessibility and cross-species differences in animal models. Here we establish a human midbrain model that uses hiPSC-derived midbrain organoids and apply it to assess cell-type-specific responses to acute and chronic fentanyl treatment and fentanyl withdrawal. Single-cell mRNA sequencing of 25,510 cells from organoids in different treatment groups indicated that chronic fentanyl treatment arrests neuronal subtype specification during early midbrain development and alters synaptic activity and neuron projection. In contrast, acute fentanyl treatment increases dopamine release but does not significantly alter gene expression related to cell lineage development. These results provide the first examination of the effects of opioid exposure on human midbrain development at the single cell level.
Published in
Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids
Kim HS, Xiao Y, Chen X et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 · PMID 38549185 · doi:10.1002/advs.202400847
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Also filed as BioProject PRJNA1082742 and SRA study SRP492865. Searching any of these in the dataset finder brings you back here.

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