DNA accessibility sites requiring SWI/SNF or β-catenin activity [ATAC-seq]

To reveal the effects of β-catenin knockout or acute SWI/SNF (BAF) inhibition on chromatin accessibility, we performed knockout of β-catenin or treated cells with 1 µM of BAF inhibitor BRM014 or DMSO vehicle control and performed ATAC-seq. Analysis of altered DNA accessibility in H295R cells revealed that sites with Steroidogenic Factor-1 (SF-1) motifs had the strongest loss of DNA accessibility. In HEK 293T cells, sites with motifs belonging to β-catenin binding partners (specifically YAP binding-partner TEAD, SOX, or FOXO motifs) had loss of DNA accessibility upon either BAF inhibition or β-catenin knockout.