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Deciphering the role of RNA in regulating CTCF’s DNA binding affinity in leukemia cells [ATAC-seq]

GSE261179 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/03/26 Platform GPL24676
Summary
CTCF is the most studied transcription factor, which is essential for chromatin interaction maintenance. Although several independent studies reported that CTCF protein could pull down RNAs in vitro and in cells, there are continuous debates about authenticity of the RNA-binding affinity of CTCF and its biological role, mainly due to the concerns of limited research techniques such as CLIP-seq. To systematically investigate the RNA-interaction with CTCF and the impact on gene regulation, we conducted in vivo CTCF ChIP-seq under three conditions, including RNase A treatment, Triptolide-mediated transcription inhibition and auxin-inducible degron mediated RNA-binding domain mutant swap. Our results consistently suggest that when RNA-interaction with CTCF protein was impaired in vivo, the DNA occupancy of CTCF remains the same at the genome-wide scale. Our data provide a complementary approach and in silico evidence to re-considerate the role of RNA-binding affinity of CTCF.
Published in
Deciphering the role of RNA in regulating CTCF's DNA binding affinity in leukemia cells
Hyle J, Qi W, Djekidel MN et al. · Genome biology 2025 · PMID 40355969 · doi:10.1186/s13059-025-03582-x
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Direct links to NCBI, no account and no request form: the whole study as GSE261179_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1085695 and SRA study SRP494173. Searching any of these in the dataset finder brings you back here.

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