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A negatively-charged region within C-terminal domain maintains proper CTCF DNA binding (ATAC-Seq)

GSE261213 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/11/30 Platform GPL24676
Summary
As an essential regulator of higher-order chromatin structures, CTCF is a highly conserved DNA binding protein with a central DNA-binding domain of 11 tandem zinc fingers, which are flanked by N- and C-terminal domains of intrinsically disordered regions. The N-terminal domain interacts with cohesin complex and the central ZF domains recognize a large range of diverse genomic sites. However, the function of C-terminal unstructured domain remains less understood. Here, we found that deletion of C-terminal fragment of 116 amino acid increases CTCF-DNA binding. By genetic dissection of this fragment, we uncovered a negatively-charged region responsible for repressing CTCF binding to DNA. Thus, the unstructured C-terminal domain play an important role in maintaining proper CTCF-DNA interactions.
Published in
A negatively charged region within carboxy-terminal domain maintains proper CTCF DNA binding
Liu L, Tang Y, Zhang Y et al. · iScience 2024 · PMID 39720519 · doi:10.1016/j.isci.2024.111452
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Also filed as BioProject PRJNA1073239. Searching any of these in the dataset finder brings you back here.

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