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Genome-wide studies identify the role of PML/RARα phase separation in PML/RARα and BRD4 chromatin occupancy

GSE261292 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2024/12/31 Platform GPL16791
Summary
Acute promyelocytic leukemia (APL) is characterized by a specific t(15;17) chromosome translocation that generates the promyelocytic leukemia/retinoic acid receptor-α (PML/RARα) fusion gene. However, the global association between PML/RARα and transcriptional co-regulators, and the rules of their association in governing the key processes during the leukemogenesis remain unclear. Here, we performed the genome-wide binding profiling of PML/RARα and BRD4 in NB4, an APL patient-derived cell line. Moreover, we also performed ChIP-seq of PML/RARα and BRD4 upon genetic or pharmacological pertubation of PML/RARα or BRD4 to determine how they target regulatory elements.
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Direct links to NCBI, no account and no request form: the whole study as GSE261292_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1086441 and SRA study SRP494466. Searching any of these in the dataset finder brings you back here.

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