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Early Prediction of Preeclampsia Based on Transposable Elements Deregulation in the Placenta [RNA-Seq]

GSE261306 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2026/07/01 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
30 samples: 15 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Preeclampsia (PE) is a pregnancy-associated hypertension disorder which affects 5–10% of pregnant women each year worldwide, which leads to adverse maternal and child outcomes. PE remains inadequately predicted, prevented and treated. Here we investigated the epigenetic alterations contributing to PE. We found an increase in histone acetylation levels across transposable elements (TEs) in the preeclamptic placenta.
Read the full summary ↓
Histone hyperacetylation in PE is associated with an increased expression of endogenous retrovirus (ERV) and long interspersed nuclear element 1 (L1, LINE1) subfamilies of TEs in five independent pregnancy cohorts. A higher level of TE RNAs correlates with an elevated type I interferon (IFN-I) pathway, suggesting inflammation associated with PE could be due to the sensing of TE transcripts by the antiviral innate immune system. Furthermore, TE-focused analysis of maternal cell-free RNA (cfRNA) data revealed differential levels of TE subfamilies in PE compared to healthy controls. Training TE transcript signatures for PE prediction showed a robust performance of area under the curve (AUC) of 0.96, with validation cohort AUC of 0.88 with 81% sensitivity and 74% positive predictive value (PPV). Overall, these findings show TE deregulation is associated with PE pathology and analysis of TE levels in maternal cfRNA is an exciting approach for early diagnosis of PE, which can improve prophylaxis, obstetric and neonatal outcomes.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE261306_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1086462 and SRA study SRP494521. Searching any of these in the dataset finder brings you back here.

Study design
15 conditions, mostly in duplicate
Placenta, RNA Control1 ×2 Placenta, RNA PE2 ×2 Placenta, RNA PE+IUGR1 ×2 Placenta, RNA Control2 ×2 Placenta, RNA PE1 ×2 Placenta, RNA Control3 ×2 Placenta, RNA PE3 ×2 Placenta, RNA PE+IUGR2 ×2 +7 more

Supports a between-group comparison across 30 samples.

How we read this: 15 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.