Effect of Hypoxia, Oscillatory Hypoxia, and Lactate on MDA 231 Cells
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × MDA 231 cells, Control vs 3 × MDA 231 cells, Hypoxia vs 3 × MDA 231 cells, Oscillatory Hypoxia vs 3 × MDA 231 cells, LactateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Lactate in breast cancer cells is associated with evasion of hypoxia-induced cell cycle arrest and adverse patient outcomeHuman cell · 2024
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE261312_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1086471 and SRA study SRP494524. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8140385 MDA 231 cells, Control 1 MDA 231 cells, Control
- GSM8140386 MDA 231 cells, Control 2 MDA 231 cells, Control
- GSM8140387 MDA 231 cells, Control 3 MDA 231 cells, Control
- GSM8140388 MDA 231 cells, Hypoxia 1 MDA 231 cells, Hypoxia
- GSM8140389 MDA 231 cells, Hypoxia 2 MDA 231 cells, Hypoxia
- GSM8140390 MDA 231 cells, Hypoxia 3 MDA 231 cells, Hypoxia
- GSM8140391 MDA 231 cells, Oscillatory Hypoxia 1 MDA 231 cells, Oscillat…
- GSM8140392 MDA 231 cells, Oscillatory Hypoxia 2 MDA 231 cells, Oscillat…
- GSM8140393 MDA 231 cells, Oscillatory Hypoxia 3 MDA 231 cells, Oscillat…
- GSM8140394 MDA 231 cells, Lactate 1 MDA 231 cells, Lactate
- GSM8140395 MDA 231 cells, Lactate 2 MDA 231 cells, Lactate
- GSM8140396 MDA 231 cells, Lactate 3 MDA 231 cells, Lactate
- GSE292924 Effects of Normoxia, Hypoxia, Oscillatory Hypoxia, and Lactate on Wild-Type, NOTCH1-Knockdown, and TP53-Knockdown MDA-MB-231 Cells 36 samples shares mda, oscillatory, hypoxia, lactate
- GSE268804 Effect of depletion of hypoxia-inducible factor (HIFs) on gene expression in MDA231-BrM2 breast cancer brain-selective metastatic cells with RNA-Seq. 12 samples shares mda, hypoxia
- GSE297242 Effect of lactate accumulation on translation and transcription in MDA-MB-231 breast cancer cells 12 samples shares mda, lactate
- GSE228336 Hypoxia and lactate influence VOC production in A549 lung cancer cells 108 samples shares hypoxia, lactate
- GSE266011 Effect of CRY2 stabilizer and proteasome inhibitor on the transcription of MDA-MB-231 cells [RNA-seq] 36 samples shares mda
- GSE254862 Effect of ADAR1 and MDA5 knock down by siRNA on human SMC phenotypic modulation and calcification in vitro 42 samples shares mda
- GSE251710 MDA-MB-468 cells treated with docetaxel or EZH2i+AKTi 36 samples shares mda
- GSE336730 RNA-seq analysis of MDA-MB-231 and MDA-MB-453 cells treated with abemaciclib and/or fulvestrant 24 samples shares mda
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.