The impact of PCR duplication on RNAseq data generated using NovaSeq 6000, NovaSeq X, AVITI and G4 sequencers
RNA-seq (gene expression) · human · 144 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 144 samples: 144 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- The impact of PCR duplication on RNAseq data generated using NovaSeq 6000, NovaSeq X, AVITI, and G4 sequencersGenome biology · 2025
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE261432_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1086933 and SRA study SRP494838. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 144 sample titles: 144 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8143919 Element AVITI liver, mRNA derived cDNA, 0ng input, 17 PCR cycles
- GSM8143920 Element AVITI liver, mRNA derived cDNA, 0ng input, 19 PCR cycles
- GSM8143921 Element AVITI liver, mRNA derived cDNA, 0ng input, 21 PCR cycles
- GSM8143922 Element AVITI liver, mRNA derived cDNA, 1ng input, 16 PCR cycles
- GSM8143923 Element AVITI liver, mRNA derived cDNA, 1ng input, 18 PCR cycles
- GSM8143924 Element AVITI liver, mRNA derived cDNA, 1ng input, 20 PCR cycles
- GSM8143925 Element AVITI liver, mRNA derived cDNA, 2ng input, 15 PCR cycles
- GSM8143926 Element AVITI liver, mRNA derived cDNA, 2ng input, 17 PCR cycles
- GSM8143927 Element AVITI liver, mRNA derived cDNA, 2ng input, 19 PCR cycles
- GSM8143928 Element AVITI liver, mRNA derived cDNA, 4ng input, 14 PCR cycles
- GSM8143929 Element AVITI liver, mRNA derived cDNA, 4ng input, 16 PCR cycles
- GSM8143930 Element AVITI liver, mRNA derived cDNA, 4ng input, 18 PCR cycles
- GSM8143931 Element AVITI liver, mRNA derived cDNA, 7ng input, 13 PCR cycles
- GSM8143932 Element AVITI liver, mRNA derived cDNA, 7ng input, 15 PCR cycles
- GSM8143933 Element AVITI liver, mRNA derived cDNA, 7ng input, 17 PCR cycles
- GSM8143934 Element AVITI liver, mRNA derived cDNA, 15ng input, 12 PCR cycles
- GSM8143935 Element AVITI liver, mRNA derived cDNA, 15ng input, 14 PCR cycles
- GSM8143936 Element AVITI liver, mRNA derived cDNA, 15ng input, 16 PCR cycles
- GSM8143937 Element AVITI liver, mRNA derived cDNA, 31ng input, 11 PCR cycles
- GSM8143938 Element AVITI liver, mRNA derived cDNA, 31ng input, 13 PCR cycles
- GSM8143939 Element AVITI liver, mRNA derived cDNA, 31ng input, 15 PCR cycles
- GSM8143940 Element AVITI liver, mRNA derived cDNA, 62ng input, 10 PCR cycles
- GSM8143941 Element AVITI liver, mRNA derived cDNA, 62ng input, 12 PCR cycles
- GSM8143942 Element AVITI liver, mRNA derived cDNA, 62ng input, 14 PCR cycles
- GSM8143943 Element AVITI liver, mRNA derived cDNA, 125ng input, 9 PCR cycles
- GSM8143944 Element AVITI liver, mRNA derived cDNA, 125ng input, 11 PCR cycles
- GSM8143945 Element AVITI liver, mRNA derived cDNA, 125ng input, 13 PCR cycles
- GSM8143946 Element AVITI liver, mRNA derived cDNA, 250ng input, 8 PCR cycles
- GSM8143947 Element AVITI liver, mRNA derived cDNA, 250ng input, 10 PCR cycles
- GSM8143948 Element AVITI liver, mRNA derived cDNA, 250ng input, 12 PCR cycles
- GSM8143949 Element AVITI liver, mRNA derived cDNA, 500ng input, 7 PCR cycles
- GSM8143950 Element AVITI liver, mRNA derived cDNA, 500ng input, 9 PCR cycles
- GSM8143951 Element AVITI liver, mRNA derived cDNA, 500ng input, 11 PCR cycles
- GSM8143952 Element AVITI liver, mRNA derived cDNA, 1000ng input, 6 PCR cycles
- GSM8143953 Element AVITI liver, mRNA derived cDNA, 1000ng input, 8 PCR cycles
- GSM8143954 Element AVITI liver, mRNA derived cDNA, 1000ng input, 10 PCR cycles
- GSM8143955 G4 liver, mRNA derived cDNA, 0ng input, 17 PCR cycles
- GSM8143956 G4 liver, mRNA derived cDNA, 0ng input, 19 PCR cycles
- GSM8143957 G4 liver, mRNA derived cDNA, 0ng input, 21 PCR cycles
- GSM8143958 G4 liver, mRNA derived cDNA, 1ng input, 16 PCR cycles
+ 104 more — browse all 144 samples with per-sample file links →
- GSE310267 OR7A10 GPCR engineering boosts CAR-NK therapy against solid tumors 36 samples shares pcr
- GSE274397 Manifold roles of CCR7 chemokine GPCR in human trophoblast differentiation 28 samples shares pcr
- GSE267153 Systems modeling of oncogenic G-protein and oncogenic GPCR signaling reveals unexpected differences in downstream signaling pathway activation [FAKi_experiment] 27 samples shares pcr
- GSE290019 Endocytosis sculpts distinct cAMP signal transduction by endogenously coexpressed GPCRs 18 samples shares pcr
- GSE282963 Human endothelial colony forming cells (ECFCs) require endothelial protein C receptor (EPCR) for cell cycle progression and angiogenic activity 12 samples shares pcr
- GSE312291 A Novel Function of the Branched-Chain Fatty Acid iso15:0 in Suppressing HCC Malignant Progression through GPCR/PI3K/AKT Pathway Inhibition 10 samples shares pcr
- GSE267152 Systems modeling of oncogenic G-protein and oncogenic GPCR signaling reveals unexpected differences in downstream signaling pathway activation [CY_GQ_experiment] 9 samples shares pcr
- GSE270457 The Role of Orphan GPCR GPR153 in Regulating Vascular Damage Responses 9 samples shares pcr
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.