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A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [scRNA-Seq]

GSE262416 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2026/01/21 Platform GPL24247
Summary
Decreasing fertility rates has emerged as a significant social and medical concern, with male infertility accounting for at least half of the cases. Conventional semen analysis offers restricted prognostic utility for male fertility, largely because a considerable number of male infertility incidents are attributed to dysfunctions in the testicular interstitium. Here we have not only characterized transcriptome data and reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitor, but also examined super enhancers information in both young, adult and aged mice Leydig cell progenitors. Our findings reveal A comprehensive atlas of testicular interstitium during aging in both H3K27ac modifications and gene expression alterations. Specifically, the transcriptional activities of key genes involved in progenitor stemness appear to be regulated by super-enhancers. These discoveries offer pivotal insights for developing novel cell-based therapies to ameliorate testicular dysfunction in older individuals.
Published in
A single-cell atlas of the testicular interstitium defines Leydig progenitor networks sustaining Leydig cell homeostasis across the lifespan
Huang X, Xia K, Yang M et al. · eLife 2025 · PMID 41433200 · doi:10.7554/eLife.100396
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Also filed as BioProject PRJNA1091684 and SRA study SRP497747. Searching any of these in the dataset finder brings you back here.

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