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LARP3, LARP7, and MePCE are Involved in the Early Stage of Human Telomerase RNA Biogenesis

GSE262590 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/07/25 Platform GPL34284
Summary
Human telomerase assembly is a highly dynamic process. Using biochemical approaches, we found that LARP3 and LARP7/MePCE are involved in the early stage and that their binding to hTR is destabilized when the mature hTR is produced. LARP3 plays a negative role in preventing the processing of the 3′-extended long (exL) form and binding of LARP7 and MePCE. Interestingly, the tertiary structure of the exL form prevents LARP3 binding and facilitates hTR biogenesis. Supporting this process, LARP3 at low levels promotes hTR maturation, increases telomerase activity, and elongates telomeres. LARP7 and MePCE knockdown inhibits the conversion of the 3′-extended short (exS) form into mature hTR and the cytoplasmic accumulation of hTR, resulting in telomere shortening. Our data suggest that LARP3 and LARP7/MePCE mediate the processing of hTR precursors and thus control the production of functional telomerase.
Published in
LARP3, LARP7, and MePCE are involved in the early stage of human telomerase RNA biogenesis
Kao TL, Huang YC, Chen YH et al. · Nature communications 2024 · PMID 39009594 · doi:10.1038/s41467-024-50422-w
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Also filed as BioProject PRJNA1092608 and SRA study SRP498132. Searching any of these in the dataset finder brings you back here.

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