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Cardiac differentiation roadmap for analysis of plasticity and balanced lineage commitment

GSE263326 Homo sapiens Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/01/27 Platform GPL18573
Summary
Stem cell-based models of human heart tissue and cardiac differentiation employ monolayer and 3D organoid cultures with different properties, cell type composition, and maturity. Here we show how cardiac monolayer, embryoid body, and engineered heart tissue trajectories compare in a single-cell roadmap of atrial and ventricular differentiation conditions. Using a multiomic approach and gene-regulatory network inference, we identified regulators of the epicardial, atrial and ventricular cardiomyocyte lineages. We identified ZNF711 as a regulatory switch and safeguard for cardiomyocyte commitment. We show that ZNF711 ablation prevents cardiomyocyte differentiation in the absence of retinoic acid, causing progenitors to be diverted more prominently to epicardial and other lineages. Retinoic acid rescues this shift in lineage commitment and promotes atrial cardiomyocyte differentiation by regulation of shared and complementary target genes, showing an interplay between ZNF711 and retinoic acid in cardiac lineage commitment.
Published in
Cardiac differentiation roadmap for analysis of plasticity and balanced lineage commitment
Snabel RR, Cofiño-Fabrés C, Baltissen M et al. · Stem cell reports 2025 · PMID 40020683 · doi:10.1016/j.stemcr.2025.102422
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Also filed as BioProject PRJNA1096712 and SRA study SRP500042. Searching any of these in the dataset finder brings you back here.

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