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Glial differentiation of mouse embryonic-stem-cell-derived neuronal precursors is a sensitive marker for assessing developmental neurotoxicity of insecticides

GSE263683 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2025/04/21 Platform GPL24247
Summary
Many epidemiological and animal exposure studies have suggested that exposure to environmental substances is a major risk factor for developmental neurotoxicity (DNT), such as in autism, and is related to the increasing relevance of neurodevelopmental disorders. Recent efforts have led to the development of various in vitro approaches that use cell lines and pluripotent stem cells to assess numerous environmental substances. In this study, we developed a method for assessing DNT using a mouse embryonic stem (mES) cell model that focuses on differentiation into neuronal cells (neural cells and astrocytes). Using this model system, we found that six insecticides inhibited the differentiation of neural precursor cells into astrocytes. Our data indicated that the effects of insecticides on glial differentiation were more sensitive than those of several DNT markers reported in previous studies. This mES cell model can make a quick assessment of DNT potential and may be a useful tool for screening substances with potential to induce DNT.
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Direct links to NCBI, no account and no request form: the whole study as GSE263683_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1098738 and SRA study SRP500822. Searching any of these in the dataset finder brings you back here.

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