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Human Early Postnatal Thymus [ATAC-seq]

GSE264585 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2024/08/30 Platform GPL30173
Summary
Within the thymus, regulation of the cellular cross-talk directing T cell development is dependent on spatial interactions within specialized niches. To create a holistic, spatially defined map of tissue niches guiding postnatal T cell development we employed the multidimensional imaging platform CO-detection by indEXing (CODEX), as well as Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) and Assay for Transposable-Accessible Chromatin (ATAC-seq). We generated age-matched 4–5-month-old postnatal thymus datasets for both male and female donors, and describe signaling and cell-cell interaction networks in sequential thymocyte developmental niches. Together, these data represent a unique age-matched spatial multiomic resource to investigate how sex-based differences in thymus regulation and T cell development arise, and provide an essential resource to understand the mechanisms underlying immune function and dysfunction in males and females.
Published in
Sex-biased human thymic architecture guides T cell development through spatially defined niches
Stankiewicz LN, Salim K, Flaschner EA et al. · Developmental cell 2025 · PMID 39383865 · doi:10.1016/j.devcel.2024.09.011
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Direct links to NCBI, no account and no request form: the whole study as GSE264585_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1103192 and SRA study SRP503410. Searching any of these in the dataset finder brings you back here.

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