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Single-cell ATAC-seq analysis of mouse embryonic stem cells before and after Cohesin depletion

GSE266089 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/05/01 Platform GPL30172
Summary
The contrast between the disruption of genome topology upon cohesin loss and the lack of downstream gene expression changes instigates intense debates regarding the structure-function relationship between genome and gene regulation. Here, by analyzing transcriptome at the single-cell level, we discover that, instead of dictating population-wide gene expression levels, cohesin supplies a general function to neutralize stochastic co-expression tendency of cis-linked genes in single cells. Notably, through single-cell ATAC-seq analysis of mouse embryonic stem cells, we found that cohesin loss induces chromatin co-opening tens of million bases apart in cis. Our results support that cohesin arranges nuclear topology to control gene co-expression in single cells.
Published in
Cohesin prevents cross-domain gene coactivation
Dong P, Zhang S, Gandin V et al. · Nature genetics 2024 · PMID 39048795 · doi:10.1038/s41588-024-01852-1
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Also filed as BioProject PRJNA1105866 and SRA study SRP504566. Searching any of these in the dataset finder brings you back here.

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