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A novel immunosenescent CD8+ T cell subset in patients with Ankylosing Spondylitis and Psoriatic Arthritis links spontaneous motility to telomere shortening and dysfunction

GSE266295 Homo sapiens (human) RNA-seq (gene expression) 16 samples Submitted 2025/07/09 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
16 samples: 8 × migrated vs 8 × not_migratedGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
An Immunosenescent CD8+ T Cell Subset in Patients with Axial Spondyloarthritis and Psoriatic Arthritis Links Spontaneous Motility to Telomere Shortening and DysfunctionArthritis & rheumatology (Hoboken, N.J.) · 2025
Summary (written by the submitters)
Objectives: A pathogenetic role of CD8+ T lymphocytes in ankylosing spondylitis (AS) and other spondyloarthritis (SpA) is sustained by genome-wide association studies (GWAS) as well as the expansion of public T cell clonotypes in the target tissues. This study focuses on CD8+ T cells from AS and psoriatic arthritis (PsA) patients with the aim to correlate their phenotype and function with motility.
Read the full summary ↓
Methods: Peripheral blood CD8+ and CD4+ T cells were isolated from AS (n= 128), PsA (n= 60) and rheumatoid arthritis (RA, n= 74) patients and healthy donors (HD, n= 79). Transwell migration assay was performed in the presence of different chemokines. CD8+ T cell immunoprofiling and effector functions were assessed by multiparametric flow cytometry. Transcriptome signature was evaluated by RNA-seq analysis while telomere length and dysfunction were measured by RT-PCR and IF-fluorescence in situ hybridization (FISH), respectively. Results: A significantly higher number of CD8+ T cells migrating in the absence of chemokine stimuli was found in patients with SpA compared to HD and RA patients. This subset, producing cytotoxic (granzyme B, perforin, granulysin) and inflammatory molecules (TNFα), was significantly enriched in terminally differentiated (CCR7-CD45RA+) and senescent (CD28-CD57+) cells with a gene expression profile characterized by cytolytic signature and natural killer (NK) markers. Of note, these spontaneously migrating CD8+ T cells showed activation of DNA damage response (DDR), telomere shortening and dysfunction. Conclusions: Our data describe a terminally differentiated CD8+ T cell subset with a senescent and cytotoxic/proinflammatory profile and an intrinsic invasive potential enriched in SpA patients that represents a possible player in disease pathogenesis.
Published in
An Immunosenescent CD8+ T Cell Subset in Patients with Axial Spondyloarthritis and Psoriatic Arthritis Links Spontaneous Motility to Telomere Shortening and Dysfunction
Paldino G, Tedeschi V, Proganò V et al. · Arthritis & rheumatology (Hoboken, N.J.) 2025 · PMID 39835465 · doi:10.1002/art.43109
This dataset
Open GSE266295 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE266295_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1106519 and SRA study SRP505009. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39835465) ↗
Study design
8 × migrated vs 8 × not_migrated

Supports a between-group comparison across 16 samples.

How we read this: 2 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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