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Multiomics analysis of differentiating human cholangiocyte organoids

GSE267024 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/12/10 Platform GPL24676
Summary
We aim to uncover the molecular mechanisms driving liver cellular plasticity in human chronic liver disease. We first derived intrahepatic cholangiocytes organoids (ICOs). The resulting organoid lines were then differentiated into biphenotypic cells expressing hepatocytes markers mimicking a process occurring during chronic injury in vivo. We then utilised multi-omics single nuclei RNA sequencing (snRNAseq) and assased for transcriptomics signatures and transposase-accessible chromatin (ATAC) expression to uncover the molecular pathways and the epigenetic regulations required for the in vitro conversion of cholangiocytes into hepatocytes.These analyses allowed for the identification of transcription factors involved in this process. Our analysis also suggested transdifferentiation could represent the main process by which hepatocytes are produced during liver regeneration.
Published in
Transcriptomic and epigenetic mechanisms controlling cholangiocyte transdifferentiation into hepatocytes
Galanakis V, Gribben C, Munteanu A et al. · Journal of hepatology 2026 · PMID 41033615 · doi:10.1016/j.jhep.2025.09.021
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Also filed as BioProject PRJNA1109364 and SRA study SRP506550. Searching any of these in the dataset finder brings you back here.

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