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Autoimmune inflammation causes hematopoietic stem cells to generate a trained immunity program inherited by BMDMs [ATACseq_primary_data]

GSE267516 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/10/15 Platform GPL24247
Summary
Trained immunity occurs when inflammatory stimulation reprograms myeloid cells to have increased effector functions upon secondary stimulation. We found that autoimmune inflammation indiced trained immunity in bone marrow derived macrophages (BMDMs) in mice treated with pristane for eight weeks. We then analyzed the chromatin landscape and transcriptome using low input chromatin accessibility and transcriptomics sequencing (LiCAT-seq) which allows for the co-generation of RNA-seq and ATAC-seq libraries from the same cellular pool. With this approach we found that BMDMs had increased chromatin accessibility at metabolic genes and inflammatory related genes in BMDMs from pristane treated mice, which resulted in increased transcription of metabolic and inflammatory genes, leading to enhanced metabolism and inflammatory functions in BMDMs.
Published in
A distinct metabolic and epigenetic state drives trained immunity in HSC-derived macrophages from autoimmune mice
Mills TS, Kain B, Burchill MA et al. · Cell stem cell 2024 · PMID 39413777 · doi:10.1016/j.stem.2024.09.010
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Also filed as BioProject PRJNA1111762 and SRA study SRP507762. Searching any of these in the dataset finder brings you back here.

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