Mechanical stretch induces senescence of alveolar epithelial cells and drives fibroblast activation by paracrine mechanisms
RNA-seq (gene expression) · human · 25 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 25 samples: 6 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Mechanical Stretch Induces Senescence of Lung Epithelial Cells and Drives Fibroblast Activation by Paracrine MechanismsAmerican journal of respiratory cell and molecular biology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE267788_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1112870 and SRA study SRP508409. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 25 samples.
How we read this: 6 replicated groups read from 25 sample titles; they account for 25 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8278693 A549_stretch_Senescent_1 A549_stretch_Senescent
- GSM8278694 A549_stretch_Non_senescent_2 A549_stretch_Non_senesc…
- GSM8278695 A549_stretch_Senescent_3 A549_stretch_Senescent
- GSM8278696 A549_stretch_Senescent_4 A549_stretch_Senescent
- GSM8278697 A549_stretch_Senescent_5 A549_stretch_Senescent
- GSM8278698 A549_stretch_Non_senescent_6 A549_stretch_Non_senesc…
- GSM8278699 A549_stretch_Non_senescent_7 A549_stretch_Non_senesc…
- GSM8278700 A549_stretch_Non_senescent_8 A549_stretch_Non_senesc…
- GSM8278707 A549_doxo_1 A549_doxo
- GSM8278708 A549_doxo_2 A549_doxo
- GSM8278709 A549_doxo_3 A549_doxo
- GSM8278710 A549_stretch_senescent_4 A549_stretch_senescent
- GSM8278711 A549_stretch_senescent_5 A549_stretch_senescent
- GSM8278712 A549_stretch_senescent_6 A549_stretch_senescent
- GSM8278713 A549_stretch_senescent_7 A549_stretch_senescent
- GSM8278832 MRC5_static_1 MRC5_static
- GSM8278833 MRC5_stretch_2 MRC5_stretch
- GSM8278834 MRC5_static_3 MRC5_static
- GSM8278835 MRC5_stretch_4 MRC5_stretch
- GSM8278836 MRC5_static_5 MRC5_static
- GSM8278837 MRC5_stretch_6 MRC5_stretch
- GSM8278838 MRC5_static_7 MRC5_static
- GSM8278839 MRC5_stretch_8 MRC5_stretch
- GSM8278840 MRC5_static_9 MRC5_static
- GSM8278841 MRC5_stretch_10 MRC5_stretch
- GSE275647 Role of Cancer-Associated Fibroblasts in Prognostic Stratification of Advanced-Stage Gastric Cancer through Interacting with Endothelial and Malignant Epithelial Cells [AGC RNA-seq] 216 samples shares epithelial, fibroblast
- GSE273882 Arginine Promotes The Activation of Human Lung Fibroblasts Independent of Its Metabolism 52 samples shares fibroblast, activation
- GSE295260 CDK4/6 inhibition induces a senescence-associated secretory phenotype via delayed NF-κB activation 40 samples shares senescence, activation
- GSE298023 Location and etiology modulate fibroblast activation in the failing human heart 18 samples shares fibroblast, activation
- GSE275648 Role of Cancer-Associated Fibroblasts in Prognostic Stratification of Advanced-Stage Gastric Cancer through Interacting with Endothelial and Malignant Epithelial Cells [AGC scRNA-seq] 11 samples shares epithelial, fibroblast
- GSE289881 Exploring fibroblast activation protein as an early biomarker in chronic lung allograft dysfunction 10 samples shares fibroblast, activation
- GSE235244 Senescent cancer-associated fibroblasts in pancreatic adenocarcinoma suppress CD8+ T cell activation and inhibit response to immune checkpoint therapy [hPDAC] 8 samples shares fibroblast, activation
- GSE307326 Continuous NRTI-based antiretroviral therapy induces progressive senescence-like reprogramming of alveolar macrophages [snMultiomics] 32 samples shares senescence, alveolar
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.