← BioTransfer GEO Dataset Finder
GEO series

StealTHY: enabling CRISPR-Cas9 screens in immunocompetent hosts to expose concealed metastatic regulators [paired-end]

GSE268045 Mus musculus (mouse) RNA-seq (gene expression) 70 samples Submitted 2025/10/29 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 70 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
70 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
StealTHY: An immunogen-free CRISPR platform to expose concealed metastasis regulators in immunocompetent modelsCell · 2025
Summary (written by the submitters)
CRISPR screens have become standard gene discovery platforms in various contexts, including cancer. Yet, commonly available CRISPR-Cas9 tools are increasingly recognized as unfit for in vivo investigations in immunocompetent contexts, due to the broad immunogenicity of bacterial nucleases and reporters. Here, we show how CRISPR screens with tumor grafts are systematically jeopardized by immunoediting of Cas9-expressing cells in immunocompetent hosts, resulting in iatrogenic clonal dropouts and ultimately compromising target identification.
Published in
StealTHY: An immunogen-free CRISPR platform to expose concealed metastasis regulators in immunocompetent models
Saini M, Castro-Giner F, Hotz A et al. · Cell 2025 · PMID 41205600 · doi:10.1016/j.cell.2025.10.007
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE268045_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1114094 and SRA study SRP509055. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 70 more — browse all 70 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.