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The lncRNA DUBR is regulated by CTCF and coordinates chromatin landscape and gene expression in hematopoietic cells [ChIP-Seq]

GSE268500 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 24 samples Submitted 2024/05/28 Platform GPL16791
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 24 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
24 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The lncRNA DUBR is regulated by CTCF and coordinates chromatin landscape and gene expression in hematopoietic cellsNucleic acids research · 2025
Summary (written by the submitters)
Master hematopoietic transcription factors (TFs) and long non-coding RNAs (lncRNAs) coordinate shaping lineage-specific gene expression programs during hematopoietic differentiation. The architectural protein CCCTC-binding factor (CTCF) has emerged as a pivotal regulator of gene expression in cell differentiation. However, the relationship and its regulatory effect of CTCF on lncRNA genes in hematopoiesis remains elusive.
Read the full summary ↓
We demonstrated that CTCF constrains DUBR transcription throughout erythroid differentiation. DUBR is highly expressed in human hematopoietic stem and progenitor cells (HSPC) but depleted in erythroblasts. DUBR perturbation dysregulates the expression of hematopoietic-erythroid cell differentiation genes and facilitates genome-wide activation of regulatory elements. A genomic map of RNA occupancy revealed that DUBR directly regulates a set of TFs involved in regulating hematopoietic differentiation, including the erythroid repressor HES1, which in turn targets a subset of regulatory elements of DUBR-dysregulated genes. Our results support the role of DUBR as a regulator of a hematopoietic differentiation gene program, by coordinating the expression of TFs and influencing the chromatin regulatory landscape.
Published in
The lncRNA DUBR is regulated by CTCF and coordinates chromatin landscape and gene expression in hematopoietic cells
Núñez-Martínez HN, Tapia-Urzúa G, Cerecedo-Castillo ÁJ et al. · Nucleic acids research 2025 · PMID 39995041 · doi:10.1093/nar/gkaf093
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE268500_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1117467 and SRA study SRP510311. Searching any of these in the dataset finder brings you back here.

Samples in this study

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