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GABARAPs proteins modulate FVIII secretion in molecule-specific manner via energy related pathways

GSE269206 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2026/06/30 Platform GPL16791
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
30 samples: 30 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Little is known about the biogenesis of Factor VIII (FVIII), its control of expression, intracellular trafficking, secretion, and associated cellular energy requirements. In this project, we employed a Y2H approach in to identify new interacting proteins that may play a role in the above mentioned processes. As a result, we successfully isolated GABARAP, an ATG8 protein, as a potential interactor with the FVIII-B domain.
Read the full summary ↓
While GABARAP did not co-immunoprecipitate with FVIII, we observed a weak interaction with full-length FVIII (FL-FVIII) but not with the B-domain-deleted FVIII (BDD-FVIII) using the BLITZ system. Additionally, there was a significant increase in the co-localization of GABARAP with FL-FVIII compared to BDD-FVIII. Furthermore, both transient knockdown of GABARAP through siRNA and stable knockout using CRISPR resulted in a decrease in FVIII secretion. Similar effects on FVIII secretion were observed for knockouts of MCFD2, LMAN1, and Calreticulin, while a contrasting effect, increasing secretion, was seen for knockouts of Calnexin, GABARAPL1, GABARAPL2, and ATG7. To explain the differential effects on FVIII secretion, we examined the expression profiles of the knockouts and the wild-type clones, revealing differences in several pathways, notably energy related oxidative phosphorylation and mitochondrial function. The residual cellular ATP content and cellular mitochondrial function showed a strong correlation with FVIII secretion levels. These findings establish a link between GABARAP proteins, the cellular energy status, and FVIII secretion.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269206_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1120569 and SRA study SRP512083. Searching any of these in the dataset finder brings you back here.

Study design
30 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 30 sample titles: 30 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.