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PP1 PNUTS binds the “restrictor” and dephosphorylates pol II CTD Ser5 to stimulate RNA pol II transcription termination [nascent RNA-seq]

GSE269357 Homo sapiens (human) RNA-seq (gene expression) 21 samples Submitted 2025/04/05 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 21 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
21 samples: 7 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
PP1/PNUTS phosphatase binds the restrictor complex and stimulates RNA Pol II transcription terminationCell reports · 2025
Summary (written by the submitters)
The restrictor complex, WDR82/ZC3H4, is the major termination factor for antisense transcription from bidirectional promoters, but its mechanism of action is poorly understood. We report that the PP1 phosphatase nuclear targeting subunit PNUTS binds to restrictor via its WDR82 subunit. AlphaFold predicts a quaternary complex, PPWZ, in which PP1-associated PNUTS and ZC3H4 both contact WDR82.
Read the full summary ↓
A chimera comprising inactive PP1H66K fused to C-terminal PNUTS sequences is a dominant-negative inhibitor of antisense termination and CTD Ser5 dephosphorylation and both activities require the PNUTS WDR82 binding domain. PP1H66K-PNUTS is a substrate trap that also interacts with pol II large subunit and nuclear exosome components. Ser5 hyperphosphorylated pol II has increased processivity and pauses less frequently than total pol II. We propose that the elevated elongation efficiency of Ser5-P pol II antagonizes early termination and that Ser5 dephosphorylation by PP1/PNUTS/WDR82/ZC3H4 is coupled to termination of antisense transcription.
Published in
PP1/PNUTS phosphatase binds the restrictor complex and stimulates RNA Pol II transcription termination
Erickson B, Fedoryshchak R, Fong N et al. · Cell reports 2025 · PMID 40244850 · doi:10.1016/j.celrep.2025.115564
This dataset
Open GSE269357 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269357_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1121272 and SRA study SRP512458. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40244850) ↗
Study design
7 conditions, mostly in duplicate
HEK293 PP1(H66K)-PNUTS_pdox_BruSeq ×4 HEK293 PP1(H66K)-PNUTS_mdox_BruSeq ×3 HEK293 PP1(H66K)-PNUTSDIPLD_pdox_BruSeq ×3 HEK293 PP1(WT)-PNUTS_pdox_BruSeq ×2 HEK293 PP1(H66K)-PNUTS_mdox_DRB15'BruSeq ×2 HEK293 PP1(H66K)-PNUTS_pdox_DRB15'BruSeq ×2 HEK293 PP1(H66K)-PNUTSDWBD_pdox_BruSeq ×2 HEK293 PP1(WT)-PNUTS_mdox_BruSeq ×1 +2 more

Supports a between-group comparison across 18 samples.

How we read this: 7 replicated groups read from 21 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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