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Effects of oxidized LDL versus IL-1ß/TNF-ɑ/INFɣ on human gingival mesenchymal stem cells properties

GSE269628 Homo sapiens (human) RNA-seq (gene expression) 18 samples Submitted 2025/02/26 Platform GPL15520
At a glance

RNA-seq (gene expression) · human · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
18 samples: 18 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Effects of oxidized LDL versus IL-1ß/TNF-ɑ/INFɣ on human gingival mesenchymal stem cells propertiesJournal of periodontal research · 2025
Summary (written by the submitters)
Oxidized low-density lipoprotein (oxLDL) is an important player in the course of metabolic inflammatory diseases. oxLDL was identified in the gingival crevicular fluid, denoting possible associations between oxLDL-induced inflammation and periodontal disease. The current investigation compared direct effects of oxLDL to a cytokine cocktail of IL-1ß/TNF-ɑ/INF-γ on gingival mesenchymal stem cells’ (G-MSCs) attributes.
Read the full summary ↓
G-MSCs exhibited all mesenchymal stem cells’ characteristics. oxLDL- and cytokine-group displayed no disparities in their stemness markers (p>0.05). Next-generation-sequencing revealed altered expression of the TXNIP gene in response to oxLDL treatment compared to controls (p=0.04). Following an initial boosting for up to 5 days by inflammatory stimuli, over 14 day, cellular counts [median count x10-5(Q25/Q75)] were utmost in control- [2.6607(2.0804/4.5357)], followed by cytokine- [0.0433(0.0026/1.4215)] and significantly lowered in the oxLDL-group [0.0274(0.0023/0.7290);p=0.0047). Osteogenic differentiation [median relative Ca2⁺ content(Q25/Q75)] was significantly lower in cytokine- [0.0066(0.0052/0.0105)] compared to oxLDL- [0.0144(0.0108/0.0216)] (p=0.0133), with no differences notable for chondrogenic and adipogenic differentiation (p>0.05). Within the current investigation’s limitations, in contrast to cytokine mediated inflammation, G-MSCs appear to be minimally responsive to oxLDL-mediated metabolic inflammation, with little effect on their regenerative attributes.
Published in
Effects of oxidized LDL versus IL-1ß/TNF-ɑ/INFɣ on human gingival mesenchymal stem cells properties
Fawzy El-Sayed K, Mahlandt E, Schlicht K et al. · Journal of periodontal research 2025 · PMID 38952262 · doi:10.1111/jre.13319
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE269628_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1122789 and SRA study SRP513286. Searching any of these in the dataset finder brings you back here.

Study design
18 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 18 sample titles: 18 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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