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Effect of depletion of IGF2BP3 on gene expression in LUAD

GSE270702 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/04/09 Platform GPL24676
Summary
IGF2BP3, an oncofetal protein and an m6A reader, is a member of the IGF2BPs family playing an important role in cell migration in early embryogenesis, oncogenesis, metabolism and metastasis. To clarify the effect of depletion of IGF2BP3 on gene expression in LUAD, we conducted IGF2BP3 knocked out experiments in the NCI-H1299 LUAD cell lines. The knockout efficiency was validated by western blot analysis, demonstrating successful silencing of IGF2BP3 at the protein levels in NCI-H1299 cells. To explore the underlying mechanisms of IGF2BP3 in LUAD development, we conducted RNA-sequencing analysis using IGF2BP3 knockout and control NCI-H1299 cells, with each group consisting of three biological replicates. The analysis revealed that IGF2BP3 knockout resulted in differential expression of 1700 genes including 621 genes upregulated and 1079 downregulated.
Published in
IGF2BP3 Triggers STAT3 Pathway by Stabilizing SRC RNA in an m6A-Dependent Manner to Promote Lymphatic Metastasis in LUAD
Ding J, Wang X, Yang H et al. · Cancer science 2025 · PMID 39805702 · doi:10.1111/cas.16451
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Also filed as BioProject PRJNA1128042 and SRA study SRP515960. Searching any of these in the dataset finder brings you back here.

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