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Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3 [10X]

GSE270917 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2024/07/02 Platform GPL24676
Summary
We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow via robotic implementation and established best practices to consistently achieve high cell capture efficiency and data quality. In comparison with the 10X platform, HT Smart-seq3 analysis of primary CD4+ T-cells demonstrated superior sensitivity in gene detection and similar capability to capture major cellular heterogeneity upon sufficient scaling up. Notably, through T-cell receptor (TCR) reconstruction, HT Smart-seq3 identified more productive pairs of alpha and beta chains without additional primer design, enabling more comprehensive profiling of TCRs. Collectively, HT Smart-seq3 provides a cost-effective and scalable method for characterization of single-cell transcriptomes and immune repertoires.
Published in
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
Chuang HC, Li R, Huang H et al. · BMC genomics 2024 · PMID 39574011 · doi:10.1186/s12864-024-11036-0
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Also filed as BioProject PRJNA1129021 and SRA study SRP516513. Searching any of these in the dataset finder brings you back here.

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