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Cytoplasmic translocation of tumor suppressor HINT1 and its impact on cell shape during density transition

GSE270956 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/07/14 Platform GPL24676
Summary
Cells at low density exhibit spreading and migration behaviors with more spread-out morphologies, while high-density cultures show confined, rounded morphologies. During this transition, the actin cytoskeleton rearranges from oriented stress fibers to a cortical actin shell, supporting cell movement and shape maintenance. This transition also halts cell proliferation to prevent uncontrolled growth, as seen in tumors. However, the molecular mechanism behind actin cytoskeleton remodeling by cell density is not well understood. Using proteomics of purified open chromatin, we identified the tumor suppressor HINT1 as a key molecule whose nucleocytoplasmic shuttling depends on cell density. RNA-seq analysis of wild-type (WT) and knock-out (KO) HEK293A cells revealed that HINT1 is involved in morphogensis and development.
Published in
The nucleocytoplasmic translocation of HINT1 regulates the maturation of cell density
Zhang X, Nakamura F · Life science alliance 2025 · PMID 40695554 · doi:10.26508/lsa.202503215
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Also filed as BioProject PRJNA1129113 and SRA study SRP516560. Searching any of these in the dataset finder brings you back here.

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