Impacts of Glut1 Deficiency and High Glucose Exposure on Transcriptomic Profiling in Human Brain Microvascular Endothelial Cells
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × HGNS vs 3 × HGSiG vs 3 × NS vs 3 × SiGGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Glucose transporter 1 is essential to maintain brain endothelial cell homeostasis under hyperglycemia conditionAmerican journal of physiology. Cell physiology · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE271637_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1132537 and SRA study SRP518301. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8380955 HGNS_rep1 HGNS
- GSM8380956 HGNS_rep2 HGNS
- GSM8380957 HGNS_rep3 HGNS
- GSM8380958 HGSiG_rep1 HGSiG
- GSM8380959 HGSiG_rep2 HGSiG
- GSM8380960 HGSiG_rep3 HGSiG
- GSM8380961 NS_rep1 NS
- GSM8380962 NS_rep2 NS
- GSM8380963 NS_rep3 NS
- GSM8380964 SiG_rep1 SiG
- GSM8380965 SiG_rep2 SiG
- GSM8380966 SiG_rep3 SiG
- GSE267498 Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies 37 samples shares microvascular, endothelial, brain
- GSE281329 Time-course Transcriptomics Reveals Impact of Treponema pallidum on Microvascular Endothelial Cell Function and Phenotype 39 samples shares microvascular, endothelial
- GSE292538 KRAS-Dependent Metabolic Reprogramming of Endothelial Cells as a Therapeutic Vulnerability in Brain Arteriovenous Malformations 18 samples shares endothelial, brain
- GSE299978 Accelerated Reprogramming of hiPSCs into Functional Brain Endothelial Cells with Multiplexed CRISPR Activation 11 samples shares endothelial, brain
- GSE275647 Role of Cancer-Associated Fibroblasts in Prognostic Stratification of Advanced-Stage Gastric Cancer through Interacting with Endothelial and Malignant Epithelial Cells [AGC RNA-seq] 216 samples shares endothelial
- GSE254249 Remodeling of T and endothelial cells during total neoadjuvant therapy in rectal cancer 92 samples shares endothelial
- GSE294621 Vascular-type heterogeneity is associated with differential gene expression profiles of endothelial cells under shear stress 74 samples shares endothelial
- GSE303084 Pressure Points: Endothelial Responses to Hemodynamic Stress in Health and Pulmonary Arterial Hypertension 72 samples shares endothelial
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.