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RLS-associated MEIS transcription factors control distinct processes in human neural stem cells [ChIP-Seq]

GSE271673 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/12/03 Platform GPL21697
Summary
MEIS1 and MEIS2 encode highly conserved homeodomain transcription factors crucial for developmental processes in a wide range of tissues, including the brain. They can execute redundant functions when co-expressed in the same cell types, but their roles during early stages of neural differentiation have not been systematically compared. By separate knockout and overexpression of MEIS1 and MEIS2 in human neural stem cells, we find they control specific sets of target genes, associated with distinct biological processes. Integration of DNA binding sites with differential transcriptomics implicates MEIS1 to co-regulate gene expression by interaction with transcription factors of the SOX and FOX families. MEIS1 harbors the strongest risk factor for restless legs syndrome (RLS). Our data suggest that MEIS1 can directly regulate the RLS-associated genes NTNG1, MDGA1 and DACH1, constituting new approaches to study the elusive pathomechanism or RLS.
Published in
RLS-associated MEIS transcription factors control distinct processes in human neural stem cells
Kittke V, Zhao C, Lam DD et al. · Scientific reports 2024 · PMID 39578497 · doi:10.1038/s41598-024-80266-9
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Also filed as BioProject PRJNA1132898 and SRA study SRP518459. Searching any of these in the dataset finder brings you back here.

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