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Effect of Knockdown of PAXBP1 on gene expression of HaCaT keratinocytes

GSE272102 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/07/01 Platform GPL16791
Summary
Here, we report that the targeted deletion of Paxbp1 in epidermal keratinocytes mediated by Keratin14-Cre leads to severe disruption in skin architecture. Mice deficient in Paxbp1 exhibited a substantially reduced epidermal thickness and pronounced separation at the dermo-epidermal junction upon birth. Mechanistically, we demonstrate that the absence of Paxbp1 hinders cellular proliferation, marked by a halt in cell cycle transition, suppressed gene expression of proliferation, and a compromised DNA replication pathway in basal keratinocytes, resulting in the thinning of skin epidermis. Moreover, molecules and pathway associated with hemidesmosome assembly were impaired in Paxbp1-deficient keratinocytes, culminating in the detachment of the skin epidermal layer. Therefore, our study highlights an indispensable role of Paxbp1 in the maintenance of epidermal homeostasis.
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Also filed as BioProject PRJNA1135120 and SRA study SRP519544. Searching any of these in the dataset finder brings you back here.

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