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Effect of compounds (9, 13, 20 and 21) on the HIV-1 transcription in TZM-bl cells infected with HIV-1NL4-3

GSE273184 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/01 Platform GPL24676
Summary
We developed a cellular screening system capable of simultaneously evaluating the functional activities of Tat-induced LTR transcription and general cellular expression. Herein, we identified and optimized novel HIV-1 Tat inhibitory compounds that contain an oxadiazole core. To assess the inhibitory effect of our lead compounds on Tat-mediated HIV-1 transcription, we performed RNA-seq analyses of the HIV-1 transcripts in the compound-treated HIV-1-infected cells. The read counts of the transcripts mapped to the HIV-1 genome were substantially reduced in the compound 9- or 13-treated cells; however, the decrease of the read counts was not observed in the HIV-1 infected cells with the ineffective compounds (20 and 21). These data indicate that the viral transcription step is a target for our lead compounds.
Published in
Discovery of new acetamide derivatives of 5-indole-1,3,4-oxadiazol-2-thiol as inhibitors of HIV-1 Tat-mediated viral transcription
Shin Y, Park CM, Kim D-E et al. · Antimicrobial agents and chemotherapy 2024 · PMID 39230310 · doi:10.1128/aac.00643-24
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Also filed as BioProject PRJNA1140571 and SRA study SRP522423. Searching any of these in the dataset finder brings you back here.

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