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METTL3 alters capping enzyme expression and its activity on ribosomal proteins in NSCLC cell model

GSE273305 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/10/21 Platform GPL11154
Summary
The 5’ cap, catalyzed by capping enzyme, RNGTT, is a vital mRNA modification for the functionality of mRNAs and dysregulated in cancer cells. The m6A writer METTL3 is a regulator of mRNA stability and protein translation, and its expression is altered in various cancers, including lung cancer cells. We identified a METTL3 motif in the mRNA of RNGTT and investigated whether METTL3 could regulate the expression of RNGTT. Knock down of METTL3 resulted in destabilizing RNGTT mRNA, reduced protein expression and activity. Sequencing of capped mRNAs and m7G immunoprecipitation identified an underrepresentation of ribosomal protein mRNA.
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Also filed as BioProject PRJNA1141406 and SRA study SRP522782. Searching any of these in the dataset finder brings you back here.

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