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lncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2 [ChIP-seq]

GSE273480 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/11/18 Platform GPL24676
Summary
Organisms regulate cell size and shape to function efficiently. Aberrant cell morphogenesis is commonly associated with disease, yet gene-regulatory mechanisms remain unknown. CISTR-ACT was the first lncRNA involved in inter-chromosomal contacts and Mendelian disease, and is associated with mean corpuscular volume (red blood cell size). Here, functional dissection of CISTR-ACT’s DNA- and RNA-encoded mechanisms by in vitro and in vivo perturbations reveal that CISTR-ACT regulates cell size across cell types and species. CISTR-ACT’s locus is embedded in a stable inter-chromosomal environment which contains cell size genes that are regulated by CISTR-ACT in trans. CISTR-ACT’s RNA also has function and directly interacts with transcription factor FOSL2 to guide its regulation of cell morphogenesis and cell-cell adhesion genes. In the absence of CISTR-ACT, the FOSL2-chromatin binding is perturbed. Our study exemplifies how a functionally conserved lncRNA regulates cell size with multiple modes of action and ultimately contributes to clinically relevant phenotypes.
Published in
LncRNA CISTR-ACT regulates cell size in human and mouse by guiding FOSL2
Kiriakopulos K, Soleimanpour K, McMurray BJ et al. · Nature communications 2025 · PMID 41397992 · doi:10.1038/s41467-025-67591-x
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Also filed as BioProject PRJNA1141992 and SRA study SRP523171. Searching any of these in the dataset finder brings you back here.

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