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Effect of APC depletion on gene expression in JEG-3 cells

GSE273945 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/01/01 Platform GPL16791
Summary
Fetal-maternal immune tolerance guarantees a successful pregnancy throughout gestation. HLA-G, a non-classical human leukocyte antigen (HLA) molecule exclusively expressed in extravillous trophoblasts (EVT), is a crucial factor in establishing fetal-maternal immune tolerance by interacting with inhibitory receptors on various maternal immune cells residing in the uterus. While trophoblast specific Cis-Regulatory Elements (CREs) impacting HLA-G transcription have been described, the identity of trans-acting factors controlling HLA-G expression in EVT remains poorly understood. Utilizing a genome-wide CRISPR-Cas9 knockout screen, we find that the WNT signaling pathway negatively regulates HLA-G expression in EVT. APC is a key component of the destruction complex for cytoplasmic β-catenin, switching off the canonical WNT pathway. This dataset containes the transcriptomics in JEG-3 cells upon APC knockout.
Published in
The TEA domain transcription factors TEAD1 and TEAD3 and WNT signaling determine HLA-G expression in human extravillous trophoblasts
Gu B, Ferreira LMR, Herrera S et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 40096597 · doi:10.1073/pnas.2425339122
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Also filed as BioProject PRJNA1144136 and SRA study SRP524132. Searching any of these in the dataset finder brings you back here.

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