← BioTransfer GEO Dataset Finder
GEO series

METTL3-dependent m6A RNA methylation represses transposable elements derived enhancers to suppress naïve pluripotency in human pluripotent stem cells [RNA-Seq]

GSE274217 Homo sapiens (human) DNA methylation 28 samples Submitted 2025/04/16 Platform GPL20301
At a glance

DNA methylation · human · 28 samples

What it is
DNA methylationChemical marks on DNA that help switch genes on or off.
Organism
Homo sapiens (human)
Samples
28 samples: 14 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
METTL3-dependent m6A RNA methylation regulates transposable elements and represses human naïve pluripotency through transposable element-derived enhancersNucleic acids research · 2025
Summary (written by the submitters)
Using a METTL3 inducible knockout (iKO) system in human expanded potential stem cells (hEPSCs), we uncovered that unlike in mESCs, METTL3 was indispensable for hPSCs’ maintenance, and that loss of METTL3 caused significant upregulation of naïve pluripotency genes accompanied with impaired hEPSCs differentiation particularly towards to both trophoblastic and amnionic lineage. Mechanistically, METTL3 iKO in hPSCs substantially increased expression of two primate-specific transposable elements (TEs), SVA_D and HERVK/LTR5_Hs.
Published in
METTL3-dependent m6A RNA methylation regulates transposable elements and represses human naïve pluripotency through transposable element-derived enhancers
Zhang W, Fu H, Huang Y et al. · Nucleic acids research 2025 · PMID 40298111 · doi:10.1093/nar/gkaf349
This dataset
Open GSE274217 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274217_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for beta-value tables or raw IDAT files among the supplementary files.

Also filed as BioProject PRJNA1145416 and SRA study SRP524751. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40298111) ↗
Study design
14 conditions, mostly in duplicate
METTL3 iKO hEPSC Clone 1 with DOX, ×2 METTL3 iKO hEPSC Clone 1 in Passage 2 without… ×2 METTL3 iKO hEPSC Clone 1 in Passage 3 without… ×2 METTL3 iKO hEPSC Clone 2 with DOX, ×2 METTL3 iKO hEPSC Clone 2 in Passage 2 without… ×2 METTL3 iKO hEPSC Clone 2 in Passage 3 without… ×2 METTL3 iKO primed hESC Clone 1 with DOX, ×2 METTL3 iKO primed hESC Clone 1 in Passage 2 w… ×2 +6 more

Supports a between-group comparison across 28 samples.

How we read this: 14 replicated groups read from 28 sample titles; they account for 28 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.