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Modulation of Gene Expression by Substrate Stiffness via Regulation of Histone H2B Mono-ubiquitination

GSE274425 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/03/26 Platform GPL20795
Summary
Mechanotransduction plays a critical role in regulating cell growth, proliferation, and metabolism. Cells respond to mechanical signals, which ultimately induces gene expression. However, the underlying mechanisms of this gene regulation remain largely unclear. In previous research, we discovered that the ubiquitin-conjugating enzymes UBE2A and UBE2B translocate between the cytoplasm and nucleus in a force-dependent manner. Additionally, we confirmed that UBE2A and UBE2B ubiquitinate histone H2B at lysine 120 (K120) when cells are cultured on a stiff substrate. In this study, we employed Chromatin Immunoprecipitation (ChIP) to investigate the distribution of H2B mono-ubiquitination in human skeletal muscle (hsSKM) cells cultured on substrates of varying stiffness—soft (0.2 kPa) and stiff (64 kPa).
Published in
Modulation of Gene Expression by Substrate Stiffness via Ubiquitination of Histone H2B by Ubiquitin-Conjugating Enzyme E2A/B
Feng M, Nakamura F · ACS omega 2025 · PMID 40290990 · doi:10.1021/acsomega.5c02459
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Also filed as BioProject PRJNA1146391 and SRA study SRP525379. Searching any of these in the dataset finder brings you back here.

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