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In vivo Spatial Perturb-Seq in the mouse brain

GSE274447 Mus musculus (mouse) Other 3 samples Submitted 2025/12/01 Platform GPL28330
At a glance

mouse · 3 samples

Organism
Mus musculus (mouse)
Samples
3 samples: 2 × MouseGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI.
Paper
Spatial perturb-seq: single-cell functional genomics within intact tissue architectureNature communications · 2026
Summary (written by the submitters)
Functional genomics that knocks out individual genes is ideal for investigating gene functions. Examining these perturbations in vivo, within native tissue architecture and with spatial context, is crucial for accurately assessing the impact of genetic modifications both within the cell and on its microenvironment.
Published in
Spatial perturb-seq: single-cell functional genomics within intact tissue architecture
Shen K, Seow WY, Keng CT et al. · Nature communications 2026 · PMID 41723140 · doi:10.1038/s41467-026-69677-6
This dataset
Download · which file do I need?

Direct links to NCBI, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need.

All supplementary files ↗
3 · The original raw files

Whole study · RAW.tar

Everything the authors deposited as one archive (GSE274447_RAW.tar), including the raw array or instrument files. Can be large; the picker in step 2 takes just the samples you need.

Also filed as BioProject PRJNA1146440. Searching any of these in the dataset finder brings you back here.

Study design
2 × Mouse

Supports a between-group comparison across 2 samples.

How we read this: 1 replicated groups read from 3 sample titles; they account for 2 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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