EHMT1 mediates cellular motility in embryonal rhabdomyosarcoma by activating SOX8 expression.
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Topic
- Soft tissue sarcomaThis series is part of our curated briefing on it.
- Samples
- 12 samples: 6 × scrambled siRNA, vs 3 × EHMT1-specific siRNA, vs 3 × SOX8-specific siRNA,Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE274482_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1146484 and SRA study SRP525462. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 3 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8449972 scrambled siRNA, replicate 1 scrambled siRNA,
- GSM8449973 scrambled siRNA, replicate 2 scrambled siRNA,
- GSM8449974 scrambled siRNA, replicate 3 scrambled siRNA,
- GSM8449975 EHMT1-specific siRNA, replicate 1 EHMT1-specific siRNA,
- GSM8449976 EHMT1-specific siRNA, replicate 2 EHMT1-specific siRNA,
- GSM8449977 EHMT1-specific siRNA, replicate 3 EHMT1-specific siRNA,
- GSM8450917 scrambled siRNA, rep 1 scrambled siRNA,
- GSM8450918 scrambled siRNA, rep 2 scrambled siRNA,
- GSM8450919 scrambled siRNA, rep 3 scrambled siRNA,
- GSM8450920 SOX8-specific siRNA, rep 1 SOX8-specific siRNA,
- GSM8450921 SOX8-specific siRNA, rep 2 SOX8-specific siRNA,
- GSM8450922 SOX8-specific siRNA, rep 3 SOX8-specific siRNA,
- GSE312030 Combined inhibition of HRAS and MEK induces tumor regression and restores myogenic differentiation in HRAS-mutant rhabdomyosarcoma 159 samples same disease briefing · shares rhabdomyosarcoma
- GSE300974 CDK8 Inhibition Releases the Muscle Differentiation Blockade in Fusion-driven Alveolar Rhabdomyosarcoma [RNA-seq] 30 samples same disease briefing · shares rhabdomyosarcoma
- GSE314949 RNA Seq of rhabdomyosarcoma cells with sgRNA targeting oncofusion interacting proteins 30 samples same disease briefing · shares rhabdomyosarcoma
- GSE279954 mRNA/sRNA Co-Sequencing Reveals Multiple New Targeted Therapy Candidates in Rhabdomyosarcoma 26 samples same disease briefing · shares rhabdomyosarcoma
- GSE292872 FANCM is Required for the PAX3::FOXO1-Driven Oncogenic Program in Rhabdomyosarcoma [RNA-Seq] 21 samples same disease briefing · shares rhabdomyosarcoma
- GSE202614 TLE3 function in rhabdomyosarcoma tumor xenografts. 20 samples same disease briefing · shares rhabdomyosarcoma
- GSE240287 RNA-sequencing of matched samples from rhabdomyosarcoma samples, obtained before therapy and mid-treatment resection 18 samples same disease briefing · shares rhabdomyosarcoma
- GSE286950 PAX3-FOXO1 drives targetable cell state-dependent metabolic vulnerabilities in rhabdomyosarcoma 16 samples same disease briefing · shares rhabdomyosarcoma
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.